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Open Access Original research

99mTc-MAG3 scintigraphy for the longitudinal follow-up of kidney function in a mouse model of renal ischemia-reperfusion injury

Tanja Herrler1, Hao Wang2, Anne Tischer1, Peter Bartenstein2, Karl-Walter Jauch1, Markus Guba1, Markus Diemling3, Cyril Nimmon4 and Marcus Hacker2*

Author Affiliations

1 Department of Surgery, Campus Großhadern, University of Munich, Munich, 81377, Germany

2 Department of Nuclear Medicine, University of Munich, Munich, 81377, Germany

3 Hermes Medical Solutions, Skeppsbron 44, Stockholm, 111 30, Sweden

4 Chiang Mai, Thailand

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EJNMMI Research 2012, 2:2  doi:10.1186/2191-219X-2-2

Published: 20 January 2012

Abstract

Background

Experimental models are essential tools in the development and evaluation of novel treatment options, but the preclinical model of renal ischemia-reperfusion injury is limited to the retrieval of (very) early functional data, leaving the pivotal long-term outcome unknown. The present study applies technetium-99m-mercapto-acetyl-tri-glycine [99mTc-MAG3] scintigraphy for the longitudinal follow-up examination of long-term kidney function after renal ischemia-reperfusion injury.

Methods

Unilateral warm ischemia was induced in scid beige mice by vascular clamping of the kidney hilum for 40 min. 99mTc-MAG3 scintigraphy was performed prior to injury, 8 and 14 days post ischemia. The fractional uptake rate [FUR] was calculated from scintigraphy data as a measure of renal clearance.

Results

FUR demonstrated a significant functional impairment of the ischemic kidney 8 and 14 days after injury (P < 0.05 vs. baseline), while contralateral non-ischemic kidneys showed no significant changes. In histological analysis, ischemic kidneys exhibited tubular dilatation and cytoplasmic degeneration as signs of hypoxia without any evidence for necrosis.

Conclusions

FUR enables the detection of renal dysfunction and longitudinal long-term follow-up examination in the same individual. Our model may facilitate preclinical therapy evaluation for the identification of effective renoprotective therapies.

Keywords:
fractional uptake rate; ischemia-reperfusion injury; 99mTc-MAG3 scintigraphy; renal clearance; tubular function