Figure 4.

Reduction in tumor vascularization after therapy. (A) 3D data analysis reveals a higher mean concentration of AngioSense in the untreated controls (control, n = 7; therapy, n = 6), P = 0.51. (B) 2D reflection data show an even stronger difference in fluorescence intensities between control and treated tumors, P = 0.19. (C) Contrast-enhanced ultrasound shows a significantly higher acoustic intensity per tumor area in control versus treated tumors (*P < 0.01). (D) Quantification of the mean vessel density demonstrates a significantly higher vessel density in the control group, **P < 0.001. (E) Immunostaining of corresponding sections confirms the markedly increased vessel number in the controls; endothelial cells are stained with CD31 (green), smooth muscle cells with SMA (red), and cell nuclei with DAPI (blue), bar = 100 μm.